Dr.Lifang Xie

HIGHLIGHTS OF EXPERTISES

  • 8 years of training in Traditional Chinese Medicine in China (5 years for a Bachelor’s Degree and 3 years for a Master’s Degree)
  • 5 years of training in Cardiovascular Pharmacology at the University of Arizona (Ph.D.)
  • Current active California Acupuncture License
  • Strong Molecular Biology background:

* Familiar with High-throughput siRNA library screening and Small molecule library screening

for novel drug target discovery

* Familiar with LC-MS/MS-based Proteomic Approach for non-invasive biomarker discovery

* Familiar with Semi-quantitative PCR, Real-time PCR, Western-blot, ELISA and

Immunocytochemistry

* Familiar with mammalian cell culture (primary/secondary)

* Familiar with cell viability assays (e.g. Cell-Titer Glow, MTT and SRB Assays)

* Familiar with Caspase Activity Assay

* Familiar with Flow cytometry

* Familiar with Matrigel Invasion Assay

  • Animal Work (mice)

* Familiar with Coronary Artery Ligation-Induced Myocardial Infarction Mouse Model

* Familiar with Doxorubicin Treatment-induced Cardiomyopathy Mouse Model

* Familiar with in vivo tumor models

  • Multitask, highly self-motivated, innovative and a strong team player
  • Strong communication skills

EDUCATION

2014~Present, Clinical Trial Certification Program, University of California Irvine, Irvine, CA

2002-2007 Ph.D., Department of Pharmacology, University of Arizona, Tucson, AZ.

Dissertation: Proteomic and Microarray Identification of Novel Cardiac Specific Indicators of Oxidative Injury and Mechanism of Action; Supervisor: Dr. Qin M. Chen, PhD.

1994-1997 M.S., Tianjin University of Traditional Chinese Medicine, Tianjin, China

          Thesis: Experimental Research on “Liver being the Root of Tolerance to Fatigue”;

           Supervisors: Dr. Yuzhou Yang and Dr. Yi Guo, PhD.

1989-1994 B.S., Hebei Medical University, Hebei, China

PROFESSIONAL EXPERIENCE

2013 – Present, Acupuncturist, Irvine, CA

2013 – 2014, Specialist, Department of Medicine, UC Irvine, Irvine, CA.

2010 – 2013, Postdoc-Employee, Department of Medicine, UC Irvine, Irvine, CA.

            Supervisor: Dr. Frank Meyskens, M.D. and F.A.C.P.

2007 – 2010, Postdoctoral fellow, Translational Genomics Research Institute (TGen); Phoenix, AZ. Supervisor: Dr. Daniel Von Hoff, M.D. and F.A.C.P.

2005 – 2007, Research Associate, Department of Pharmacology, University of Arizona.

2002 2005, Research Assistant, Pharmacology and Toxicology Program, University of Arizona.

AWARDS AND HONORS

2013 Awarded Grant from the Waltmar Foundation for Melanoma Research, Orange, CA

2012 The Best Poster Presentation Award, the 7th Conference on Metal Toxicity and Carcinogenesis, Albuquerque, NM.

2012 Travel Award, the 7th Conference on Metal Toxicity and Carcinogenesis,Albuquerque, NM.

2011 Travel Award, IPCC 2011 Conference, Bordeaux, France

2010 –2012 T32 Training Grant (PI: Wen-Hwa Lee, 5T32CA113265-04)

2007 Graduate Fellowship Award, University of Arizona Graduate College

2006 Carl C. Smith Graduate Student Awards, Honerable Mention,Mechanisms Specialty Section, Society of Toxicology (SOT)

2006 CVSS Graduate Student Poster Award, Finalist, SOT

2006 Graduate Student Travel Award, SOT

2005 Invited Presenter at the SOT 44th Annual Meeting

2003 Graduate Student Travel Award, University of Arizona

PRESENTATIONS

2012 – Poster Presentation at the 7th Conference on Metal Toxicity and Carcinogenesis

2012 – Poster Presentation at the 2012 AACR Annual meeting

2010 – Poster Presentation at the IPCC 2011 Conference

2009 – Poster Presentation at the AACR-NCI-EORTC Molecular Targets and Cancer

Therapeutics Conference

2009 – Poster Presentation at the 2009 TGen Scientific Retreat

2009 – Poster Presentation at the AACR 100th Annual Meeting

2008 – Poster Presentation at the 2008 TGen Scientific Retreat

2006 -Poster Presentation at the SOT 45th Annual Meeting

2006 -Poster Presentation at the University of Arizona Life Sciences Poster Session

2004 -Poster Presentationat the SOT 43rd Annual Meeting 

PROFESSIONAL MEMBERSHIPS

Member of the California Alliance of Acupuncture Medicine (CAAM), 2014~present

Member of the International Federation of Pigment Cell Societies (IFPCS), 2011~present

Member of the American Association for Cancer Research (AACR), 2008~present

Member of the Society of Toxicology (SOT), 2005~present

TEACHING EXPERIENCE

1996, Lecture on Foundations of Chinese Medicine for Undergraduates at Tianjin University of TCM

January 2012~present, Mentor for UC Irvine undergraduates enrolled in the Bio199 Independent

Research Program

2008~2010, Mentor for the Helios Scholars Summer Intern Program at the Translational Genomics

Research Institute (TGen, Phoenix, AZ)

EDITORIAL SERVICE

Reviewer for Life Sciences

Reviewer for Molecular Cancer Therapeutics

Reviewer for Toxicology

PUBLICATIONS

Peer-reviewed publications (*Corresponding Author)

Xie L.* and Meyskens FL Jr., The Pan-Aurora kinase Inhibitor, PHA-739358, Induces Apoptosis and Inhibits Migration in Melanoma Cell Lines. Melanoma Res. 2013 Apr; 23(2): 102-13.

Xie L., Kassner M., Munoz R., Que Q, Kiefer J., Zhao Y., Mousses S., Yin H, Von Hoff DD, and Han H, Kinome-wide siRNA Screening Identifies Molecular Targets Mediating the Sensitivity of Pancreatic Cancer Cells to Aurora Kinase Inhibitors. Biochem Pharmacol. 2012 Feb 15;83(4):452-61. Epub 2011 Nov 15.

Xie L, Terrand J, Xu B, Tsaprailis G, Boyer J, Chen QM., Cystatin C Increases in Cardiac Injury: A Role in Extracellular Matrix Protein Modulation. Cardiovasc Res. 2010 Sep 1;87(4):628-35. Epub 2010 May 20.

Xie L., Pandy R., Xu B., Tsaprailis G., Chen Q.M., Genomic and Proteomic Profiling of Oxidative Stress Response in Human Diploid Fibroblasts. Biogerontology. 2009 Apr;10(2):125-51. Epub 2008 Jul 25

Chen Q.M., Alexander D., Sun H., Xie L., Lin Y., Terrand J., Morrissy S., Purdom S., Corticosteroids Inhibit Cell Death Induced by Doxorubicin in Cardiomyocytes: Induction of Antiapoptosis, Antioxidant, and Detoxification Genes. Mol Pharmacol. 2005 Jun; 67(6):1861-73. Epub 2005 Mar 8.

Xie L., Tsaprailis G., Chen Q.M., Proteomic Identification of Insulin-like Growth Factor-binding Protein-6 Induced by Sublethal H2O2 Stress from Human Diploid Fibroblasts. Mol Cell Proteomics. 2005 Sep; 4(9):1273-1283. Epub 2005 Jun 14.

Shi L.P., Ma D.M., Xie L., Hu L.M., Wang H., Effects of Exhaustive Exercise on Liver Ultra-micro

Structure and Levels of Glycogen in Muscle and Liver—the Experimental Research on “Liver

being Root of Tolerance to Fatigue”. Liaoning Journal of Traditional Chinese Medicine. 2005,

         32(9):971-973.

Xie L., Guo Y., Wang Y.X., Yang Y., Research Progress in the Effect of Traditional Chinese Medicine

on Sports Fatigue. Chinese Journal of Sports Medicine. 1998, 17(1):67-69.

Jiao P. and Xie L., Treatment of 62 Cases of Food Retention-induced Pediatric Abdominal Pain using

          He Wei Xiao Shi Tang, Sichuan Chinese Medicine. 1995 (4): 40.

Unpublished Manuscript

Xie L.*, Angela Garcia, Liu F., Dellinger R., and Meyskens FL. Jr., Activation of c-Myc, Aurora A, Erk1/2, and NFkB signaling pathway in Cr(VI)-induced cytotoxicity in Immortalized Human Melanocytes. (Submitted to Melanoma Research).

PATENT APPLICATIONS

Haiyong Han, Ruben Munoz, Lifang Xie, Holly Yin and Daniel Von Hoff: METHODS OF ENHANCING THE ACTIVITY OF AURORA KINASE INHIBITORS. Application type: Provisional; Application number: 61092320, August 2008.

Lifang Xie and Haiyong Han: SENSITIZERS OF AURORAKINASE INHIBTORS. Application type: Provisional; Application number: 61170433, April 2009.

Inspired by tradition, pursuit with technology